If you figure out the answer for yourself, please post it!.Never delete your post, even if it has not received a response.Respond when helpful users ask follow-up questions, even if the answer is "I'm not sure".Clearly explain what you are trying to learn, not just the method used, to avoid the XY problem.Be thorough in outlining the question(s) that you are trying to answer.Image analysis is interdisciplinary, so the more general the terminology, the more people who might be able to help. Avoid discipline-specific terminology ("jargon").Screenshots (to help identify issues with tools or features).Annotated Mock-ups (showing what features you are trying to measure).Reference Images (taken from published papers).Example Images (what you want to analyze).Images give everyone a chance to understand the problem. Notes on Quality Questions & Productive Participation Here is a great macro I tried that mostly does what I'm hoping to do, but didn't work for multiple bands in one lane Īny help would be greatly appreciated, thank you! I need to do this with hundreds more samples and blots, so would like to automate this process as much as possible to get the Area and Percent of the three bands in every lane and ideally output the results to a csv. I have been using a traditional lane method of selecting each lane (Analyze > Gels > Select First/Next lane), plotting the lanes (Analyze > Gels > Plot Lanes), drawing a line to enclose the peaks, selecting the peaks, and getting the Area and Percent of each band ( Analyze > Gels > Label Peaks), which I normally copy/paste into excel. I am trying to automatically quantify the bands in a Western blot, which has three bands per lane (my protein of interest has three different forms that correspond to each band). Hello, this is my first time asking a question so I hope it's the right place! ¹This subreddit is not affiliated with the creators of ImageJ or FIJI, but is simply a place to share ideas, papers, resources, and expertise - especially as relate to questions & answers posted here. Sign-up for one of the mailing lists: /Mailing_Lists It also hosts a forum for interacting with the developers.įIJI Is Just ImageJ - "a distribution of ImageJ (and ImageJ2) together with Java, Java3D and a lot of plugins organized into a coherent menu structure." is full of ImageJ development and analysis resources. Image analysis is interdisciplinary, so clearly explain field-specific terms or jargon. Clearly explain what you are trying to learn, not just the method used, to avoid the XY problem.
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